Analytical Methods in Lipidomics and Their Applications

作者：Li, Min; Yang, Li; Bai, Yu; Liu, Huwei^*

来源：Analytical Chemistry, 2014, 86(1): 161-175.

DOI：10.1021/ac403554h

• THIN-LAYER-CHROMATOGRAPHY
• FLIGHT-MASS-SPECTROMETRY
• 2-DIMENSIONAL GAS-CHROMATOGRAPHY
• ASSISTED LASER-DESORPTION
• ENERGY COLLISIONAL DISSOCIATION
• ELECTROSPRAY-IONIZATION DESI
• POTENTIAL PLASMA BIOMARKERS
• FATTY-ACID DETERMINATION
• SOLID-PHASE EXTRACTION
• HUMAN SYNOVIAL-FLUID

As a branch of metabolomics, lipidomics is aimed at full analysis of lipid species and their biological roles with respect to health and diseases and has attracted increasing attention of biological and analytical scientists. As lipidomics has been investigated for almost 10 years, there have been several reviews about it. In this review, we focus on the recent advances in analytical methods of lipidomics, especially in the past 2 years. Herein, an overview of mass spectrometry (MS)-based methods, chromatography-based methods, and spectroscopy methods are presented. The applications of these analytical methods in several lipidomics fields are also simply discussed based on research published in the last 2 years. Lipids play multiple and critical roles in cellular functions, such as composing the membrane bilayer, providing an appropriate hydrophobic environment for membrane proteins and their interactions, and participating in cell growth, multiplication, and death. Simultaneously, some lipids are messengers in cell signaling transduction processes and can be utilized as biomarkers of some diseases.

• 出版日期2014-1-7
• 单位北京大学