Detection of Colorectal Neoplasm Using Promoter Methylation of ITGA4, SFRP2, and p16 in Stool Samples: A Preliminary Report in Korean Patients

作者：Chang Eugene; Il Park Dong^*; Kim Young Jin; Kim Bo Kyoung; Park Jung Ho; Kim Hong Joo; Cho Yong Kyun; Il Sohn Chong; Jeon Woo Kyu; Kim Byung Ik; Kim Hung Dai; Kim Dong Hoon; Kim Young Ho

来源：Hepato-Gastroenterology, 2010, 57(101): 720-727.

• Colorectal cancer
• Stool DNA
• Methylation specific PCR

Background/Aims: Colorectal cancer (CRC) screening using stool DNA has yielded a greater detection rate than conventional fecal occult blood testing. The aim of this study was to determine the sensitivity and specificity of this detection method for colorectal adenomas and carcinomas using ITGA4, SFRP2 and p16 promoter methylation.
Methods: The methylation status of ITGA4, SFRP2 and p16 promoters in bisulfite-modified stool DNA was investigated in a blinded manner with methylation specific PCR (MSP) from 31 endoscopically diagnosed healthy controls, 25 patients with adenomas and 30 patients with CRC.
Results: Methylated ITGA4, SFRP2 and p16 promoters were detected in 36.7%, 60.0%, and 40.0% of the CRC samples and in 16.0%, 44.0%, and 24.0% of the colorectal adenomas, respectively. The sensitivity of the combined study using the three markers for CRC and colorectal adenoma detection was 70.0% and 72.0%. The specificity of this method was 96.8%.
Conclusion: Our results demonstrate that ITGA4, SFRP2 and p16 promoter methylation in stool samples had high sensitivity and specificity for the detection of colorectal adenomas and CRC. This newly developed screening may be a useful non-invasive alternative screening for CRC detection.

• 出版日期2010-8