A hydrogen peroxide biosensor based on the direct electron transfer of hemoglobin in the nanosheets of exfoliated HNb3O8

作者：Liu, Yu-Ge^*; Wei, Chang-bin; Lv, Ling-Ling; Liu, Sheng-Hui

来源：Journal of Solid State Electrochemistry, 2012, 16(6): 2211-2216.

DOI：10.1007/s10008-012-1649-z

• Hemoglogin
• Direct electron transfer
• Layered HNb3O8
• Hydrogen peroxide
• Biosensor

In the present work, hemoglobin (Hb) was entrapped into the nanosheets of a pre-exfoliated layered material HNb3O8. UV-vis spectra analysis displayed that no significant denaturation occurred to the entrapped protein. Electrochemical results showed that the entrapment of Hb into layered HNb3O8 enhanced the direct electron transfer ability between protein molecules and electrode. A pair of well-defined redox peaks was observed at -0.39 and -0.34 V on the glassy carbon electrode modified with the Hb/HNb3O8 composite. The electrode reactions showed a surface-controlled process with a single electron transfer at the scan rate of 50-400 mV/s, and the electron transfer rate was very fast. The entrapped Hb retained its biological activity well and the sensor constructed by the Hb/HNb3O8-composite-modified electrode displayed excellent response to the reduction of hydrogen peroxide (H2O2) with wide linear range, low detection limit, and good stability.

• 出版日期2012-6
• 单位中国热带农业科学院