A Murine Intestinal Intraepithelial NKp46-Negative Innate Lymphoid Cell Population Characterized by Group 1 Properties

作者：Van Acker Aline; Gronke Konrad; Biswas Aindrila; Martens Lie**et; Saeys Yvan; Filtjens Jessica; Taveirne Sylvie; Van Ammel Els; Kerre Tessa; Matthys Patrick; Taghon Tom; Vandekerckhove Bart; Plum Jean; Dunay Ildiko Rita; Diefenbach Andreas; Leclercq Georges^*

来源：Cell Reports, 2017, 19(7): 1431-1443.

DOI：10.1016/j.celrep.2017.04.068

The Ly49E receptor is preferentially expressed on murine innate-like lymphocytes, such as epidermal V gamma 3 T cells, intestinal intraepithelial CD8 alpha alpha(+) T lymphocytes, and CD49a(+) liver natural killer (NK) cells. As the latter have recently been shown to be distinct from conventional NK cells and have innate lymphoid cell type 1 (ILC1) properties, we investigated Ly49E expression on intestinal ILC populations. Here, we show that Ly49E expression is very low on known ILC populations, but it can be used to define a previously unrecognized intraepithelial innate lymphoid population. This Ly49E-positive population is negative for NKp46 and CD8 alpha alpha, expresses CD49a and CD103, and requires T-bet expression and IL-15 signaling for differentiation and/or survival. Transcriptome analysis reveals a group 1 ILC gene profile, different from NK cells, iCD8 alpha cells, and intraepithelial ILC1. Importantly, NKp46(-)CD8 alpha alpha(-)Ly49E(+) cells produce interferon (IFN)-gamma, suggesting that this previously unrecognized population may contribute to Th1-mediated immunity.

• 出版日期2017-5-16
• 单位河北医科大学