A combination of naturally occurring mutations in North American West Nile virus nonstructural protein genes and in the 3 ' untranslated region alters virus phenotype

作者：Davis C Todd; Galbraith Sareen E; Zhang Shuliu; Whiteman Melissa C; Li Li; Kinney Richard M; Barrett Alan D T^*

来源：Journal of Virology, 2007, 81(11): 6111-6116.

DOI：10.1128/JVI.02387-06

We previously reported mutations in North American West Nile viruses (WNVs) with a small-plaque (sp), temperature-sensitive Qs), and/or mouse-attenuated (att) phenotype. Using an infectious clone, site-directed mutations and 3' untranslated region (3'UTR) exchanges were introduced into the WNV NY99 genome. Characterization of mutants demonstrated that a combination of mutations involving the NS4B protein (E249G) together with either a mutation in the NS5 protein (A804v) or three mutations in the YUTR (A10596G, C10774U, A10799G) produced sp, ts, and/or att variants. These results suggested that the discovery of North American WNV-phenotypic variants is rare because of the apparent requirement of concurrent pollygenic mutations.

• 出版日期2007-6