Activity of Porophyllum ruderale leaf extract and 670-nm InGaP laser during burns repair in rats

作者：Justino Jacomo Ana Cristina; Velozo Karina de Andrade; Lotti Raquel Gabilan; Grosso Neves Lia Mara; de Gaspari de Gaspi Fernanda Oliveira; Marreto Esquisatto Marcelo A; Corezola do Amaral Maria Esmeria; Sampaio Mendonca Fernanda A; Tech dos Santos Glaucia Maria

来源：BMC Complementary and Alternative Medicine, 2015, 15(1): 274.

DOI：10.1186/s12906-015-0805-2

• Porophyllum ruderale
• Laser
• Burns
• Tissue repair
• Rats

Background: In this study, we investigated the effects of an extract of the leaves of Porophyllum ruderale and laser irradiation on the healing of burns.
Methods: Seventy-two rats were divided in four groups: untreated controls, treated with laser irradiation, treated with P. ruderale and treated with both P. ruderale and laser irradiation. Burns were produced with a metal plate on the backs of the animals. Wound samples were collected for structural and morphometric analyses and to quantify the expression of TGF-beta 1 and VEGF.
Results: Laser irradiation increased the number of fibroblasts, collagen fibers and newly formed vessels and decreased the number of granulocytes at the site of the wounds. Densitometric analysis revealed a significant increase in the expression of TGF beta-1 in the wounds treated with laser irradiation and with the P. ruderale extract at the beginning of the healing process and a decreased during the experimental period. The expression of VEGF was highlighted in the lesions irradiated with laser alone.
Conclusion: Inspite of not showing a beneficial effect on the laser combination with the P. ruderale extract, when the laser was used separately, a positive effects to enhance the healing of second-degree burns was promoted. P. ruderale was effective in decreasing the granulocytes during the repair process indicating a possible anti-inflammatory action of this extract of native flora, widely used in folk medicine, but little studied experimentally.

• 出版日期2015-8-13