A novel tool for dynamic cell adhesion studies - the De-Adhesion Number Investigator DANI

作者：Hartmann Andreas; Stamp Melanie; Kmeth Ralf; Buchegger Sascha; Stritzker Bernd; Saldamli Belma; Burgkart Rainer; Schneider Matthias F; Wixforth Achim^*

来源：Lab on a Chip, 2014, 14(3): 542-546.

DOI：10.1039/c3lc50916h

For an optimal implementation of materials, such as, e.g. medical implants in living environments, a thorough characterization of cell adhesion, kinetics and strength is required, as well as a prerequisite e. g. for bone integration. Here we present a miniaturized (similar to 100 mu l) lab-on-a-chip implant hybrid system which allows quantification of cell adhesion under dynamic conditions mimicking those of physiological relevance. Surface acoustic waves are excited and used on optical transparent chips to induce micro acoustic streaming and to create a microfluidic shear spectrum ranging from 0 to similar to 35 s(-1). We demonstrate its potential for a time-efficient, dynamic screening test of new implant materials using a model of an osseointegration with SAOS-2 cells. The upside-down orientation also allows utilization of the micro reactor on non-transparent materials like titanium and diamond-like-carbon (DLC).

• 出版日期2014