科研之友
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摘要
To study the peripheral blood T-cell subsets and regulatory T-cells of multiple myeloma (MM) patients. 48 MM patients and 24 healthy controls were enrolled. Changes in peripheral blood T-cell subsets in the MM patients i.e. CD4(+)CD25(+)T cells and CD4(+)CD25(+)CD127(low) T regulatory cells (CD4(+)CD25(+)CD127(low)Tregs) and in healthy controls were measured using flow cytometry and immunohischemistry. The total T-cells (CD3(+)) in peripheral blood lymphocyte and auxiliary/induced T-cells (CD3(+)CD4(+) T cell) of the 48 MM patients showed no statistical significance when compared with those of the control group. Suppressor/cytotoxicity T-cells (CD3(+)CD8(+) T cell) increased (p < 0.05). CD4(+)CD25(+)T cells and CD4(+)CD25(+)CD127(low) Tregs were significantly higher than corresponding values in the healthy group (p < 0.05). The CD4(+)/CD8(+) T cell ratio of Stage III MM patients was significantly lower than that of the control group (p < 0.05). The CD4(+)CD25(+)T cells and CD4(+)CD25(+)CD127(low) Tregs of MM patients in the stable and the progressive stages were significantly higher than those of MM patients in the control group (p < 0.05). The abnormality of the peripheral blood T-cell subset, increased expression of CD4(+)CD25(+)CD127(low) Tregs, and low cellular immunity of MM patients are related to clinical staging and progression of the disease. The quantity of CD4(+)CD25(+)CD127(low)Tregs of peripheral blood cells of MM patients could be significantly increased through the inhibition of CD4(+) and CD8(+)T cell activities. CD4(+)CD25(+)CD127(low) Tregs promotes tumor growth through the inhibition of immunologic cell proliferation. Immunological dysfunction based on Tregs cells plays an important role in the pathogenic course.
