An overview of proteomics approaches applied to biopharmaceuticals and cyclotides research

作者：Demartini Diogo Ribeiro^*; Pasquali Giancarlo; Carlini Celia Regina

来源：Journal of Proteomics, 2013, 93: 224-233.

DOI：10.1016/j.jprot.2013.06.009

• Plant translational proteomics
• High resolution mass spectrometry
• Molecular farming
• Biopharmaceuticals
• Cyclotide
• Post-translational modification

The evolution in proteomics approaches is notable, including quantitative proteomics and strategies for elucidation of post-translational modifications. Faster and more accurate mass spectrometers as well as cleverer bioinformatics tolls are making the difference in such advancement. Among the wide range of research in plant proteomics, biopharmaceutical production using plants as "biofactories" and the screening of new activities of new molecules, in this case, peptides, are quite important regarding translational proteomics. The present review is focused on "recombinant proteins and bioactive peptides", with biopharmaceuticals and cyclotides chosen as examples. Their application and challenges are focused on a "translational proteomics" point of view, in order to exemplify some new areas of research-based on proteomics strategies.
This article is part of a Special Issue entitled: Translational Plant Proteomics.

• 出版日期2013-11-20