An immunological study of glycosaminoglycans in the connective tissue of bovine and cod skeletal muscle

作者：Hannesson Kirsten O^*; Tingbo Monica G; Olsen Ragnar L; Enersen Grethe; Baevre Anne Birgit; Ofstad Ragni

来源：Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology, 2007, 146(4): 512-520.

DOI：10.1016/j.cbpb.2006.11.024

• cattle
• fish
• glycosaminoglycans
• intramuscular connective tissue

The presence of sulfated glycosaminoglycans (GAGs) was demonstrated in the connective tissue of bovine and cod skeletal muscle by histochemical staining using Alcian blue added MgCl2 (0.06 M and 0.4 M, respectively). For further identification of the sulfated GAGs, a panel of monoclonal antibodies, 1135, 2136, 3133 and 5D4 was used that recognizes epitopes in chondroitin-0-sulfate (COS), chondroitin-4-sulfate/dermatan sulfate (C4S/DS), chondroitin-6-sulfate (C6S) and keratan sulfate (KS), respectively. Light microscopy and Western blotting techniques showed that in bovine and cod muscle COS and C6S were primarily localized pericellularly, whereas cod exhibited a more intermittent staining. C4S was expressed around the separate cells and also in the perimysium and myocommata. In contrast to bovine muscle, which hardly expressed highly sulfated KS, cod exhibited a very strong and consistent staining. Western blotting showed that COS and C6S were mainly associated with proteoglycans (PGs) of high molecular sizes in both species. Contrary to bovine muscle, C4S in cod was associated with molecules of various sizes. Both cod and bovine muscle contained KSPGs of similar sizes as C4S. KSPGs of different sizes and buoyant densities, sensitive to keratanase I and 11 were found expressed in cod.

• 出版日期2007-4