Preparation and characterization of poly(L-histidine)/poly(L-glutamic acid) multilayer on silicon with nanometer-sized surface structures

作者：Vinzenz Xenia; Hueger Erwin; Himmerlich Marcel; Krischok Stefan; Busch Sebastian; Woellenstein Juergen; Hoffmann Christian^*

来源：Journal of Colloid and Interface Science, 2012, 386(1): 252-259.

DOI：10.1016/j.jcis.2012.07.057

• Polyelectrolyte multilayer
• LbL technique
• Poly(L-histidine)
• Poly(L-glutamic acid)
• Topography
• Nanometer-sized substructure

The specific design and modification of surfaces is of great interest, especially for functional surfaces and medical applications. In order to obtain films on a surface, the layer-by-layer deposition of polyelectrolytes represents a well-established methodology. The alternating deposition of poly(L-histidine) and poly(c-glutamic acid) results in a defined, continuous surface coating that was thoroughly characterized using X-ray photoelectron spectroscopy, Fourier-transform infrared spectroscopy, ellipsometry, X-ray reflectometry, atomic force microscopy, scanning electron microscopy, contact angle, and electrokinetic measurements. Surface charge, film growth, and final thickness were measured and cross-validated. Additionally, the chemical composition and distribution of polyelectrolytes in the layerstack were determined. Finally, the optical parameters were specified and the surface topography was visualized by several methods. These characterizations revealed a coating with embedded spheroids forming from the bottom layers. This rough surface formed by (PLH/PGA)(8) was highly reproducible and might provide unique features for the design of tailored surfaces.

• 出版日期2012-11-15