Development of capillary electrophoresis - chemiluminescence detection system

作者：Tsukagoshi K^*

来源：Bunseki Kagaku, 2003, 52(1): 1-13.

DOI：10.2116/bunsekikagaku.52.1

• capillary electrophoresis
• chemiluminescence
• separation
• detection
• selectivity
• sensitivity

The author has studied capillary electrophoresis-chemiluminescence detection system. Various types of the chemiluminescence detection cells for capillary electrophoresis were developed, including flow- and batch-type cells. The author took advantage of several chemiluminescence reagents such as luminol, peroxyoxalate, ruthenium(II) complex, and 1.10-phenanthroline in the system, and also introduced several separation modes such as micellar electrokinetic chromatography, capillary isoelectric focusing, and capillary gel electrophoresis to the system. Consequently, a variety of analytes including amino acids, peptides, proteins, saccharides, nucleic acids, nucleosides, metal ions, metal complexes, alkaloides, fluorescence compounds, fluorescence compound-including liposomes, and phenolic compounds were analyzed by means of capillary electrophoresis - chemiluminescence detection system. The system provided excellent selectivity and sensitivity. The sensitivity of the system was better than those of absorption and fluorescence detection systems; for example, the detection limit for luminol was 1.5 X 10(-11) M (S/N = 3), representing the highest sensitivity of luminol yet reported. The system was applied to microchip capillary electrophoresis and immunoassay. The author also considered on peak shape in batch-type chemiluminescence detection cell for capillary electrophoresis.

• 出版日期2003-1