Elution of platinum from carboplatin-impregnated calcium sulfate hemihydrate beads in vitro

作者：Tulipan Rachel J; Phillips Heidi^*; Garrett Laura D; Dirikolu Levent; Mitchell Mark A

来源：AJVR: American Journal of Veterinary Research , 2016, 77(11): 1252-1257.

DOI：10.2460/ajvr.77.11.1252

OBJECTIVE
To characterize the elution of platinum from carboplatin-impregnated calcium sulfate hemihydrate (CSH) beads in vitro.
SAMPLE
60 carboplatin-impregnated CSH beads and 9 CSH beads without added carboplatin (controls).
PROCEDURES
Carboplatin-impregnated CSH beads (each containing 4.6 mg of carboplatin [2.4 mg of platinum]) were placed into separate 10-mL plastic tubes containing 5 mL of PBSS in groups of 1, 3, 6, or 10; 3 control beads were placed into a single tube of PBSS at the same volume. Experiments were conducted in triplicate at 37 degrees C and a pH of 7.4 with constant agitation. Eluent samples were collected at 1, 2, 3, 6, 12, 24, and 72 hours. Samples were analyzed for platinum content by inductively coupled plasma-mass spectrometry.
RESULTS
The mean concentration of platinum released per carboplatin-impregnated bead over 72 hours was 445.3 mg/L. Cumulative concentrations of platinum eluted increased as the number of beads per tube increased. There was a significant difference in platinum concentrations over time, with values increasing over the first 12 hours and then declining for all tubes. There was also a significant difference in percentage of total incorporated platinum released into tubes with different numbers of beads: the percentage of eluted platinum was higher in tubes containing 1 or 3 beads than in those containing 6 or 10 beads.
CONCLUSIONS AND CLINICAL RELEVANCE
Carboplatin-impregnated CSH beads eluted platinum over 72 hours. Further studies are needed to determine whether implantation of carboplatinimpregnated CSH beads results in detectable levels of platinum systemically and whether the platinum concentrations eluted locally are toxic to tumor cells.

• 出版日期2016-11