科研之友
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摘要
Stable isotope probing (SIP) of deoxyribonucleic acid (DNA) was used to identify microbes incorporating C-13-labeled acetate in sulfate-reducing sediment from Aarhus Bay, Denmark. Sediment was incubated in medium containing 10mM sulfate and different C-13-acetate (10, 1, 0.1mM) concentrations. The resultant changes in microbial community composition were monitored in total and SIP-fractionated DNA during long-term incubations. Chemical analyses demonstrated metabolic activity in all sediment slurries, with sulfate-reducing activity largely determined by initial acetate concentrations. Sequencing of 16S rRNA gene PCR amplicons showed that the incubations shifted the bacterial but not the archaeal community composition. After 3 months of incubation, only sediment slurries incubated with 10mM C-13-acetate showed detectable C-13-DNA labeling. Based on 16S rRNA and dsrB gene PCR amplicon sequencing, the C-13-labeled DNA pool was dominated by a single type of sulfate reducer representing a novel genus in the family Desulfobacteraceae. In addition, members of the uncultivated Crenarchaeotal group C3 were enriched in the C-13-labeled DNA. Our results were reproducible across biological replicate experiments and provide new information about the identities of uncultured acetate-consuming bacteria and archaea in marine sediments.
- 出版日期2015-8
