A rat model of bone cancer pain induced by intra-tibia inoculation of Walker 256 mammary gland carcinoma cells

作者：Mao-Ying, Qi-Liang; Zhao, Jun; Dong, Zhi-Qiang; Wang, Jun; Yu, Jin; Yan, Min-Fen; Zhang, Yu-Qiu; Wu, Gen-Cheng; Wang, Yan-Qing^*

来源：Biochemical and Biophysical Research Communications, 2006, 345(4): 1292-1298.

DOI：10.1016/j.bbrc.2006.04.186

• bone cancer pain
• Walker 256 mammary gland carcinoma cells
• ambulatory pain
• mechanical allodynia
• weight bearing difference

This study described a modified rat model of bone cancer pain. Syngeneic Walker 256 mammary gland carcinoma cells were injected into the tibia medullary cavity via intercondylar eminence. Series of tests were carried out including bone radiology, bone histology, ambulatory pain, thermal hyperalgesia, mechanical allodynia, weight bearing ability, and electrophysiological recording from primary afferent fibers. The rats inoculated with carcinoma cells showed significant ambulatory pain, mechanical allodynia, and reduction in weight bearing, as well as increased incidence of spontaneous activity in A beta fibers in affected limb, whereas PBS (vehicle) or heat-killed cells (sham) injected rats showed no significant difference in comparison to normal rats. The pain hypersensitive behaviors were aggravated with time and destruction of bone. Interestingly, mechanical allodynia was also observed in the contralateral limb, indicating the involvement of 'mirror image' pain in bone cancer pain. In summary, the present study provided a useful and easily established rat model of bone cancer pain which will contribute to further study of the mechanisms underlying cancer pain.

• 出版日期2006-7-14
• 单位上海市针灸经络研究中心; 复旦大学