Degradation of actin nucleators affects cortical polarity of aged mouse oocytes

作者：Sun, Shao-Chen; Gao, Wei-Wei; Xu, Yong-Nan; Jin, Yong-Xun; Wang, Qing-Ling; Yin, Xi-Jun; Cui, Xiang-Shun; Kim, Nam-Hyung^*

来源：Fertility and Sterility, 2012, 97(4): 984-990.

DOI：10.1016/j.fertnstert.2012.01.101

• Oocyte aging
• oocyte polarity
• actin nucleator
• actin

Objective: To investigate the molecular mechanism of mouse oocyte polarity loss during aging. @@@ Design: Experimental study. @@@ Setting: Academic basic research laboratory. @@@ Animal(s): Mice. Intervention(s): Oocytes were collected 16 hours after injection of hCG and cultured in M16 medium for an additional 14 hours with or without caffeine. @@@ Main Outcome Measure(s): Expression and localizations of actin nucleators actin-related protein 2/3 complex, JMY, and WAVE2 were examined by immunofluorescence staining, and their messenger RNA levels were examined by real-time reverse transcription-polymerase chain reaction. @@@ Result(s): The protein and messenger RNA levels of actin-related protein 2/3 complex, JMY, and WAVE2 were decreased in aged oocytes, but the levels were normal in caffeine-treated aged oocytes. @@@ Conclusion(s): Our data indicated that the loss of oocyte polarity may be due to the degradation of actin nucleators in aged oocytes. (Fertil Steril (R) 2012; 97: 984-90.

• 出版日期2012-4
• 单位延边大学