A culture-dependent survey of thermophilic bacteria from hot springs in Xiamen area in China

作者：Yang Bo; Ouyang Jianping; Ao Jingqun; Chen Xinhua^*

来源：Acta Oceanologica Sinica, 2009, 28(1): 62-71.

• Xiamen sea
• hot springs
• thermophilic bacteria
• moderately thermophilic acidophiles
• cultivable microbial community

Microbes are believed to play important roles in ecosystem function in many environments. The hot springs of Xiamen Island are close to the Xiamen Sea, and may have some characteristics different from those of inland hot springs. Microbes living in the hot springs of Xiamen may have new characteristics. However, little is known about microbial communities of hot springs close to the Xiamen Sea. A culture-dependent survey of microbial population in the Xiamen hot springs was performed by using all approach combining total cellular protein profile identification and 16S rRNA gene sequencing. A total of 328 isolates of bacteria were obtained from liquid and sediment samples from the Xiamen hot springs, including neutrophilic thermophilic bacteria and moderately thermophilic acidophiles. Neutrophilic thermophilic bacteria, which grow at a temperature range of 55-90 degrees C including Rhodothermus marinus (Strain 1), Thermus thermophilus (Strain 2), Thermus thiopara (Strain 3), Geobacillus stearothermophilus (Strain 4), Geobacillus thermoleovorans (Strain 5), and Pseudomonas pseudoalcaligenes (Strain 6), were recovered by 2216E plates. Moderately thermophilic acidophiles, which can grow at temperatures above 50 degrees C and a pH range of 1.8-3.5 such as Alicyclobacillus acidoterrestris (Strain 8), Sulfobacillus acidophilus (Strain 9), and Sufobacillus thermosulfidooxidans (Strain 10), were isolated oil selective solid medium containing sulfur and Fe(2+). Among these strains, Rhodothermus marinus, Thermus thermophilus and Geobacillus stearothermophilus are not only thermophiles, but also halophiles. One bacterium strain (Strain 6) shared 99% nucleotide sequence homology with Pseudomonas pseudoalcaligenes oil the 16S rRNA gene sequence, but was quite different from Pseudomonas pseudoalcaligenes in biological characteristics, suggesting that it may represent a novel thermophilic species. Results indicated that various species of neutrophilic thermophiles and moderately thermophilic acidophiles were widely distributed in the Xiamen hot springs and that Rhodothermus marinas and Thermus thermophilus dominated the cultivable microbial Community.

• 出版日期2009
• 单位国家海洋局第三海洋研究所