Alterations in histone acetylation following exposure to Co-60 gamma-rays and their relationship with chromosome damage in human lymphoblastoid cells

作者：Tian Xue Lei; Lu Xue; Feng Jiang Bin; Cai Tian Jing; Li Shuang; Tian Mei; Liu Qing Jie^*

来源：Radiation and Environmental Biophysics, 2018, 57(3): 215-222.

DOI：10.1007/s00411-018-0742-9

• Histone acetylation
• Chromosome damage
• Histone deacetylase inhibitor
• Suberoylanilide hydroxamic acid
• Ionizing radiation

Chromosome damage is related to DNA damage and erroneous repair. It can cause cell dysfunction and ultimately induce carcinogenesis. Histone acetylation is crucial for regulating chromatin structure and DNA damage repair. Ionizing radiation (IR) can alter histone acetylation. However, variations in histone acetylation in response to IR exposure and the relationship between histone acetylation and IR-induced chromosome damage remains unclear. Hence, this study investigated the variation in the total acetylation levels of H3 and H4 in human lymphocytes exposed to 0-2 Gy Co-60 gamma-rays. Suberoylanilide hydroxamic acid (SAHA), a histone deacetylase (HDAC) inhibitor, was added to modify the histone acetylation state of irradiated cells. Then, the total acetylation level, enzyme activity, dicentric plus centric rings (dic + r) frequencies, and micronucleus (MN) frequencies of the treated cells were analyzed. Results indicated that the acetylation levels of H3 and H4 significantly decreased at 1 and 24 h, respectively, after radiation exposure. The acetylation levels of H3 and H4 in irradiated groups treated with SAHA were significantly higher than those in irradiated groups that were not treated with SAHA. SAHA treatment inhibited HDAC activity in cells exposed to 0-1 Gy Co-60 gamma-rays. SAHA treatment significantly decreased dic + r/cell and MN/cell in cells exposed to 0.5 or 1.0 Gy Co-60 gamma-rays relative to that in cells that did not receive SAHA treatment. In conclusion, histone acetylation is significantly affected by IR and is involved in chromosome damage induced by Co-60 gamma-radiation.

• 出版日期2018-8
• 单位中国疾病预防控制中心辐射防护与核安全医学所; 中国疾病预防控制中心