Analysis of Bacterial Populations in the Environment Using Two-dimensional Gel Electrophoresis of Genomic DNA and Complementary DNA

作者：Liu Guo Hua; Nakamura Tatsuo; Amemiya Takashi; Rajendran Narasimmalu; Itoh Kiminori^*

来源：Microbes and Environments, 2011, 26(2): 184-187.

DOI：10.1264/jsme2.ME10161

• 2-DGE mapping
• 16S rRNA gene
• total and active bacterial populations
• soil
• activated sludge

Two-dimensional gel electrophoresis (2-DGE) mapping of genomic DNA and complementary DNA (cDNA) amplicons was attempted to analyze total and active bacterial populations within soil and activated sludge samples. Distinct differences in the number and species of bacterial populations and those that were metabolically active at the time of sampling were visually observed especially for the soil community. Statistical analyses and sequencing based on the 2-DGE data further revealed the relationships between total and active bacterial populations within each community. This high-resolution technique would be useful for obtaining a better understanding of bacterial population structures in the environment.

• 出版日期2011-6-1