NF-kappa B-dependent signals control BOB.1/OBF.1 and Oct2 transcriptional activity in B cells

作者：Kilzheimer Melanie; Quandt Jasmin; Langhans Julia; Weihrich Petra; Wirth Thomas; Brunner Cornelia^*

来源：European Journal of Immunology, 2015, 45(12): 3441-3453.

DOI：10.1002/eji.201545475

• B cells
• BOB.1/OBF.1 (Pou2af1)
• Oct2 (Pou2f2)
• promoter analyses
• transcriptional regulation

The transcriptional co-activator BOB.1/OBF.1 is crucial for Octamer-driven transcription in B cells. BOB.1/OBF.1-deficiency leads to tremendous defects in B-cell development and function. Therefore, in the past research focused on the identification of BOB.1/OBF.1 target genes. However, the regulation of BOB.1/OBF.1 expression itself is poorly understood. Here we show that in B cells NF-kappa B as well as to some extent NFAT proteins are involved in the activation of basal as well as inducible BOB.1/OBF.1 expression by direct binding to the BOB.1/OBF.1 promoter. Moreover, the analysis of different inducers of NF-kappa B, like several TLR ligands, TNF-alpha, BAFF, or LT alpha 1 beta 2, revealed that both the canonical and noncanonical NF-kappa B pathways are involved in the induction of BOB.1/OBF.1 gene. The identification of so far unknown inducers that regulate BOB.1/OBF.1 expression in B cells provides novel insights in the potential function of BOB.1/OBF.1 during different aspects of B-cell development and function.

• 出版日期2015-12