摘要

E. coli cells containing overexpressed (R)-selective omega-transaminase and the cofactor PLP were immobilized on methacrylate beads suitable for continuous flow applications. The use of an organic solvent suppresses leaching of PLP from the cells; no additional cofactor was required after setting up the packed-bed reactor containing the biocatalyst (omega-TA-PLP). Non-natural ketone substrates were transformed in flow with excellent enantioselectivity (%26gt;99% ee). Features of this novel system include high-throughput (30-60 min residence time), clean production (no quench, workup, or purification required), high enzyme stability (the packed-bed reactor can be continuously operated for 1-10 days), and excellent mass recovery.

  • 出版日期2014-12-5