Active roles of caspase-3 in human gastric carcinoma cell death by apoptosis inducing nucleosides from CD57(+)HLA-DRbright natural suppressor cell line

作者：Li X; Guo MW; Mori E; Mori T^*

来源：International Journal of Oncology, 2001, 18(4): 837-842.

• 57.DR-NS cell
• GCIY cell
• AIN
• apoptosis
• caspase-3

The six apoptosis inducing nucleosides (AINs), which were isolated by high performance liquid chromatograpy from CD57(+)HLA-DRheight natural suppressor (57.DR-NS) cell cultures, induced apoptosis in human gastric carcinoma (GCIY) cells demonstrating the accumulation of sub-G1 DNA content and morphological changes. By means of DNA unwinding assay, it has been revealed that DNA strand breaks were initially involved in the apoptotic cell death of GCIY cells treated with AINs followed by activation of caspase cascades, especially caspase-3. Actually, the cleavage of fluorogenic substrate for caspase-3 was identified in the reaction. Whereas, the addition of caspase-3 inhibitor into the reaction prevented the cleavage of fluorogenic substrate for caspase-3 and resulted in the blockage of the sub-G1 DNR accumulation and DNA fragmentation as apoptotic signals. Thus, it was definitely elucidated that the activation of caspase-3 displayed a key feature during AIN-induced apoptosis in GCIY cells.

• 出版日期2001-4
• 单位河北医科大学