Determination of Inorganic Anions, Organic Acids, and Amino Acids in the Common Ice Plant (Mesembryanthemum Crystallinum L.) Using Capillary Zone Electrophoresis

作者：Hattori Takanari^*; Fukushi Keiichi; Hayakawa Makoto; Minato Taro

来源：Bunseki Kagaku, 2013, 62(8): 737-741.

• amino acids
• capillary zone electrophoresis
• common ice plant
• inorganic anions
• organic acids

We developed a capillary zone electrophoresis method with direct UV detection for the determination of inorganic anions (Cl-, NO2-, and NO3-), organic acids (oxalic acid, citric acid, and malic acid), and amino acids (aspartic acid and glutamic acid) in the common ice plant (Mesembryanthemum crystallinum L.). As the background electrolyte, a 20 mmol L-1 disodium hydrogenphosphate dodecahydrate solution adjusted to pH 10.6 was used with the addition of 0.001 % (w/v) hexadimethrine bromide to reverse electroosmotic flow. The detection responses for the above analytes were improved by 3.5 - 580 fold in terms of the limits of detection (LODs, S/N = 3) in comparison with conventional indirect UV detection. The LODs for the analytes were 0.040 - 2.9 mg L-1. The values of the relative standard deviation (RSD, n = 4, intra-day) of the migration time and the peak area were, respectively, 0.081 - 0.43 % and 0.28 - 9.3 % when extract from the common ice plant was analyzed. Using the proposed procedure, Cl-, NO2-, NO3-, and oxalic acid in the common ice plant were detected within 9 min; citric acid, malic acid, aspartic acid, and glutamic acid were detected within 12 min.

• 出版日期2013-8