NSC-34 Motor Neuron-Like Cells Are Unsuitable as Experimental Model for Glutamate-Mediated Excitotoxicity

作者：Hounoum Blandine Madji; Vourc'h Patrick; Felix Romain; Corcia Philippe; Patin Franck; Gueguinou Maxime; Potier Cartereau Marie; Vandier Christophe; Raoul Cedric; Andres Christian R; Mavel Sylvie; Blasco Helene^*

来源：Frontiers in Cellular Neuroscience, 2016, 10: 118.

DOI：10.3389/fncel.2016.00118

• ALS
• Ca2+ influx
• differentiation
• NSC34
• glutamate receptors
• NMDA

Glutamate-induced excitotoxicity is a major contributor to motor neuron degeneration in the pathogenesis of amyotrophic lateral sclerosis (ALS). The spinal cord x Neuroblastoma hybrid cell line (NSC-34) is often used as a bona fide cellular model to investigate the physiopathological mechanisms of ALS. However, the physiological response of NSC-34 to glutamate remains insufficiently described. In this study, we evaluated the relevance of differentiated NSC-34 (NSC-34(D)) as an in vitro model for glutamate excitotoxicity studies. NSC-340 showed morphological and physiological properties of motor neuron-like cells and expressed glutamate receptor subunits GluA1-4, GIuN1 and GluN2A/D. Despite these diverse characteristics, no specific effect of glutamate was observed on cultured NSC-34(D) survival and morphology, in contrast to what has been described in primary culture of motor neurons (MN). Moreover, a small non sustained increase in the concentration of intracellular calcium was observed in NSC-34D after exposure to glutamate compared to primary MN. Our findings, together with the inability to obtain cultures containing only differentiated cells, suggest that the motor neuron-like NSC-34 cell line is not a suitable in vitro model to study glutamate-induced excitotoxicity. We suggest that the use of primary cultures of MN is more suitable than NSC-34 cell line to explore the pathogenesis of glutamate-mediated excitotoxicity at the cellular level in ALS and other motor neuron diseases.

• 出版日期2016-5-9