Multiplexed concentration quantification using isotopic surface-enhanced resonance Raman scattering

作者：Perera Pradeep N; Deb Shirshendu K; Davisson V Jo; Ben Amotz Dor^*

来源：Journal of Raman Spectroscopy, 2010, 41(7): 752-757.

DOI：10.1002/jrs.2513

• Raman spectroscopy
• SERRS
• IEIS
• isotope
• deuterium
• R6G
• multiplex

The recently developed isotopically edited internal standard approach for surface-enhanced resonance Raman scattering (SERRS) based chemical quantification is extended to demonstrate multiplexed detection of four different isotopic variants of a single chromophore. More specifically, it is shown that rhodamine-6G (R6G) with 0, 2, 4, or 6 deuterium substitutions may be reliably quantified in either two- or three-component mixtures. Thus, one isotopic species of known concentration may be used as an internal standard to determine the concentrations of two other isotopic components in a mixture. The concentrations of isotopic R6G SERRS chromophores are determined using partial least squares calibration and shown to yield a predictive accuracy of about +/- 10% of the total R6G concentration (over 1-50 nM concentration range). These results set the stage for the use of such isotopic variants as tags for the SERRS/SERS quantitation of mixtures containing proteins, peptides, and other compounds.

• 出版日期2010-7