Acute Carnosine Administration Increases Respiratory Chain Complexes and Citric Acid Cycle Enzyme Activities in Cerebral Cortex of Young Rats

作者：Macedo Levy W; Cararo Jose H; Maravai Soliany G; Goncalves Cinara L; Oliveira Giovanna M T; Kist Luiza W; Martinez Camila Guerra; Kurtenbach Eleonora; Bogo Mauricio R; Hipkiss Alan R; Streck Emilio L; Schuck Patricia F; Ferreira Gustavo C^*

来源：Molecular Neurobiology, 2016, 53(8): 5582-5590.

DOI：10.1007/s12035-015-9475-9

• Imidazole dipeptides
• Electron transfer chain
• Neuroprotection
• Bioenergetics

Carnosine (beta-alanyl-l-histidine) is an imidazole dipeptide synthesized in excitable tissues of many animals, whose biochemical properties include carbonyl scavenger, anti-oxidant, bivalent metal ion chelator, proton buffer, and immunomodulating agent, although its precise physiological role(s) in skeletal muscle and brain tissues in vivo remain unclear. The aim of the present study was to investigate the in vivo effects of acute carnosine administration on various aspects of brain bioenergetics of young Wistar rats. The activity of mitochondrial enzymes in cerebral cortex was assessed using a spectrophotometer, and it was found that there was an increase in the activities of complexes I-III and II-III and succinate dehydrogenase in carnosine-treated rats, as compared to vehicle-treated animals. However, quantitative real-time RT-PCR (RT-qPCR) data on mRNA levels of mitochondrial biogenesis-related proteins (nuclear respiratory factor 1 (Nrf1), peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (Ppargc1 alpha), and mitochondrial transcription factor A (Tfam)) were not altered significantly and therefore suggest that short-term carnosine administration does not affect mitochondrial biogenesis. It was in agreement with the finding that immunocontent of respiratory chain complexes was not altered in animals receiving carnosine. These observations indicate that acute carnosine administration increases the respiratory chain and citric acid cycle enzyme activities in cerebral cortex of young rats, substantiating, at least in part, a neuroprotector effect assigned to carnosine against oxidative-driven disorders.

• 出版日期2016-10