Measurement of Hanatoxin-Induced Membrane Thinning with Lamellar X-ray Diffraction

作者：Hsieh Meng Hsuan; Shiau Yu Shuan; Liou Horng Huei; Jeng U Ser; Lee Ming Tao^*; Lou Kuo Long^*

来源：Langmuir, 2017, 33(11): 2885-2889.

DOI：10.1021/acs.langmuir.7b00064

Membrane perturbation induced by cysteine-rich methodologies. Hanatoxin (HaTx), a 35-residue polypeptide peptides is a crucial biological phenomenon but scarcely investigated, in particular with effective biophysical-chemical from spider venom, works as an inhibitor of drkl (Kv2.1) channels, most likely by interacting with the voltage-sensor. However, how this water-soluble peptide modifies the gating remains poorly understood, as the voltage sensor was proposed to be deeply embedded within the bilayer. To see how HaTx interacts with phospholipid bilayers, we observe the toxin-induced perturbation on POPC/DOPG-membranes through measurements of the change in membrane thickness. Lamellar X-ray diffraction (LXD) was applied on stacked planar bilayers in the near-fully hydrated, state. The results provide quantitative evidence for the membrane thinning in a concentration-dependent manner, leading to novel and direct combinatory approaches by discovering how to investigate such a biologically relevant interaction between gating-modifier toxins and phospholipid bilayers.

• 出版日期2017-3-21
• 单位中央民族大学