Development and validation of a high-throughput method for determination of nine fluoroquinolones residues in muscle of different animal species by liquid chromatography coupled to tandem mass spectrometry with low temperature clean up

作者：Barreto Fabiano; Ribeiro Cristina B D; Hoff Rodrigo Barcellos; Dalla Costa Teresa

来源：Journal of Chromatography A, 2017, 1521: 131-139.

DOI：10.1016/j.chroma.2017.09.036

• LC-MS/MS
• Fluoroquinolones
• Sample preparation
• Poultry
• Fish
• Residues

This work describes the development and validation of a quantitative and confirmatory method to determination of nine fluoroquinolones residues in poultry, bovine, swine and fish muscle using LC-MS/MS. Sample preparation was based in a fast solvent extraction with acetonitrile with 1% of formic acid followed by a low-temperature clean up procedure and centrifugation, without further steps. The recoveries ranged between 79% and 115%. The concentration work range was 0-200 mu g kg(-1). The LOD and LOQ were Sand 10 mu g kg(-1), respectively. This high-throughput method was reliable for identification and confirmation of nine relevant compounds in food-producing animal tissues. Validation procedure was performed according the Directive 2002/657/EC criteria and the method showed fitness to purpose in terms of precision and reproducibility as well as for the other performance parameters.

• 出版日期2017-10-27