Ascorbic acid biosensor based on laccase immobilized on an electrode modified with a self-assembled monolayer and coated with functionalized quantum dots

作者：Wang Zhan; Xu Qiao; Wang Jian Hao; Yang Qin; Yu Jiu Hong; Zhao Yuan Di^*

来源：MICROCHIMICA ACTA, 2009, 165(3-4): 387-392.

DOI：10.1007/s00604-009-0149-z

• Quantum dot
• Laccase
• Ascorbic acid
• Biosensor

Laccase was immobilized on an electrode modified with a cysteine self-assembled monolayer and coated with functionalized quantum dots. The immobilized laccase is capable of directly transferring an electron. Immobilized laccase retained its activity to oxidize ascorbic acid (AA), and the apparent Michaelis-Menten constant was found to be 0.47 mM. The modified electrode was used to determine AA in the 10 to 140 mu M concentration range, with linear response curve and a detection limit of 1.4 mu M (s/n = 3).

• 出版日期2009-6
• 单位华中科技大学