Applicability and robustness of the hen%26apos;s egg test for analysis of micronucleus induction (HET-MN): Results from an inter-laboratory trial

作者：Greywe Daniela; Kreutz Juergen; Banduhn Norbert; Krauledat Matthias; Scheel Julia; Schroeder Klaus R; Wolf Thorsten; Reisinger Kerstin^*

来源：Mutation Research: Genetic Toxicology and Environmental Mutagenesis , 2012, 747(1): 118-134.

DOI：10.1016/j.mrgentox.2012.04.012

• Hen%26apos
• s egg
• Micronucleus test
• Genotoxicity
• Alternatives to animal testing
• Mutagenicity

The hen%26apos;s egg test for analysis of micronucleus formation (HET-MN) was developed several years ago to provide an alternative test system to the in vivo micronucleus test. In order to assess its applicability and robustness, a study was carried out at the University of Osnabrueck (lab A) and at the laboratories of Henkel AG %26 Co. KGaA (lab B). Following transfer of the method to lab B, a range of test substances that had been pre-tested at lab A, were tested at Henkel: the genotoxins cyclophosphamide, dimethylbenz(a)anthracene, methotrexate, acrylamide, azorubin, N-nitroso-dimethylamine and the non-genotoxins, orange G and isopropyl myristate. In a second phase, additional compounds with known in vivo properties were examined in both labs: the non-genotoxin, ampicillin, the %26quot;irrelevant%26quot; positives, isophorone and 2,4-dichlorophenol (%26quot;irrelevant%26quot; means positive in standard in vitro tests, but negative in vivo), the clastogen p-chloroaniline, and the aneugens carbendazim and vinorelbine. All substances were correctly predicted in both labs with respect to their in vivo genotoxic properties, indicating that the HET-MN may have an improved predictivity compared with current standard in vitro test systems. The results support the promising role of the HET-MN assay as a supplement to existing test batteries.

• 出版日期2012-8-30