Determination of intracellular glutathione and cysteine using HPLC with a monolithic column after derivatization with monobromobimane

作者：Conlan Xavier A^*; Stupka Nicole; McDermott Geoffrey P; Francis Paul S; Barnett Neil W

来源：Biomedical Chromatography, 2010, 24(5): 455-457.

DOI：10.1002/bmc.1327

• glutathione
• cysteine
• monobromobimane
• human skeletal muscle cells
• monolithic column chromatography

An optimized high-performance liquid chromatography (HPLC) method is used to show that, as myoblasts differentiate into multinucleated muscle fibers, there is a shift to a more oxidized cell redox state. The HPLC method incorporated derivatization with monobromobimane for the determination of the reduced (GSH) and oxidized (GSSG) forms of glutathione and the reduced (Cys) and oxidized (CysSS) forms of cysteine. The derivatization was optimized to improve the sensitivity of the approach; the limits of detection for glutathione and cysteine were 3 x 10(-8) and 5 x 10(-8) M, respectively.

• 出版日期2010-5
• 单位迪肯大学