Analysis of bean (Phaseolus vulgaris L.) proteins affected by copper stress

作者：Cuypers A^*; Koistinen KM; Kokko H; Karenlampi S; Auriola S; Vangronsveld J

来源：Journal of Plant Physiology, 2005, 162(4): 383-392.

DOI：10.1016/j.jplph.2004.07.018

• Cu
• mass spectrometry
• pathogenesis-related
• (PR) protein
• PR-10
• two-dimensional electrophoresis

The effect of excess copper on the expression of soluble proteins in 10-day old Phaseolus vulgaris seedlings was studied with two-dimensional electrophoresis and mass spectrometry, to find sensitive biochemical markers of exposure. Despite major differences in root Cu contents, both 15 and 50 μ M Cu treatments resulted in equal enhancements of Cu in the primary leaves.
Three proteins, apparently reacting in a dose-dependent manner to Cu exposure, were identified from roots. The levels of an intracellular pathogenesis-related protein and a newly identified protein homologous to PvPR1, PvPR2, were increased with increasing Cu concentration. The level of a newly identified PR-10 protein decreased in a dose-dependent manner.
No significant difference was observed in the leaf protein pattern between controls and 15 μ M Cu-treated plants. However, at 50 μ M Cu exposure, the appearance of PvPR1 and a homologue of Arabidopsis thaliana thylakoid lumenal 17.4 kDa protein was observed. Another protein slightly enhanced by Cu treatment had sequence homology to a mitochondrial precursor of glycine cleavage system H protein of Flaveria pringlei. © 2004 Elsevier GmbH.

• 出版日期2005-4