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摘要
By utilizing lipases that come from different microorganisms as catalyst, vinyl acetate as acyl donor, the asymmetric transesterification resolution of (R,S)-alpha-phenylethanol in non-aqueous media was conducted. Among the 15 enzymes investigated, lipase PS from Burkholderia cepacia showed higher activity and enantioselectivity. The effects of substrate concentration, reaction medium, enzyme dosage, reaction time, temperature, and water activity on the reaction were investigated. The results indicated that the optimal reaction conditions were as follows. The reaction was carried out with 5 mg/ml lipase PS, 0.3 mol/L (R,S)-alpha-phenylethanol, and 0.6 mol/L vinyl acetate in hexane. The mixture was then shaken under 35 degrees C and 200 r/min with the initial water activity of 0.75 for 14 h. Under the optimal conditions, the substrate conversion could reach 44.7% with 98.6% ee of (R)-phenylethyl acetate. Water activity was found to be an important factor to the product enantioselectivity and substrate conversion.
- 出版日期2011-10
- 单位江南大学
