Development of a paper-type tyrosinase biosensor for detection of phenolic compounds

作者:Senyurt Ozge; Eyidogan Fusun; Yilmaz Remziye; Oz M Tufan; Ozalp V Cengiz; Arica Yakup; Oktem Huseyin A*
来源:Biotechnology and Applied Biochemistry, 2015, 62(1): 132-136.
DOI:10.1002/bab.1246

摘要

A low-cost, portable, and disposable paper-type tyrosinase biosensor was developed for determination of phenolic compounds, using a paper-strip absorption method. Tyrosinase and a chromophore (3-methyl-2-benzothiazolinone hydrazone) were immobilized on paper strips to manufacture the biosensor, which was tested on a nontoxic substrate (l-dopamine). The biosensor was responsive to phenolic compounds such as 4-chlorophenol, catechol, m-cresol, and p-cresol. The sensor showed stability for 70days. The developed biosensor can be used for remote on-site qualitative monitoring of phenolic compounds in wastewater samples.

  • 出版日期2015-2