Degradation of chitin and production of bioactive materials by bioconversion of squid pens

作者：Wang San Lang^*; Yang Chiann Wen; Liang Tzu Wen; Peng Juo Hua; Wang Chuan Lu

来源：Carbohydrate Polymers, 2009, 78(2): 205-212.

DOI：10.1016/j.carbpol.2009.03.020

• Serratia marcescens
• Protease
• Chitosanase
• Squid pen wastes
• Deproteinization

Serratia marcescens TKU011. a protease- and chitosanase-producing bacterium, the optimized condition for protease and chitosanase production was found after the media were heated at 121 degrees C for 120 min and the culture was shaken at 25 degrees C for 5 days in 100 mL of medium containing 1% squid pen powder (SPP) (w/v), 0.1% K(2)HPO(4), and 0.05% MgSO(4). An extracellular metalloprotease with novel properties of solvent stable, and alkaline was purified from the culture supernatant of S. marcescens TKU011 with squid pen wastes as the sole carbon/nitrogen source. The enzyme was a monomeric protease with a molecular mass of 48-50 kDa by SDS-PAGE and gel filtration chromatography. The optimum pH, optimum temperature, pH stability, and thermal stability of TKU011 protease were 8, 50 degrees C, pH 5-11, and <40 degrees C, respectively. Besides protease and chitosanase, with this method, deproteinization of squid pen for beta-chitin, the production of peptide and reducing sugar may be useful for biological applications.

• 出版日期2009-9-5