A novel F1552/C1552 point mutation in the Aedes aegypti voltage-gated sodium channel gene associated with permethrin resistance

作者：Yanola Jintana; Somboon Pradya; Walton Catherine; Nachaiwieng Woottichai; Prapanthadara La aied^*

来源：Pesticide Biochemistry and Physiology, 2010, 96(3): 127-131.

DOI：10.1016/j.pestbp.2009.10.005

• Permethrin
• Resistance
• Aedes aegypti
• kdr
• Sodium channel

Sodium channel mutations were investigated through nucleotide sequencing of three cDNA fragments amplified from permethrin resistant and susceptible Aedes aegypti from northern Thailand There was a novel nucleotide substitution (T -> G) at the second position of codon 1552 resulting in the replacement of Phenylalanine by Cysteine in segment 6 domain III This amino acid was indicated by another study to involve an aromatic-aromatic contact between the sodium channel protein and the first aromatic ring of the pyrethroid alcohol moiety. Reciprocal crosses between the homozygous parental Susceptible and resistant strains indicated that resistance was autosomal and incompletely recessive, and highly associated with the homozygous mutation The bioassay of the F(2) progeny. formed by backcrossing the F(1) with the resistant parental strain, did not show a clear plateau curve across the range of doses, Suggesting that resistance to permethrin was controlled by more than one gene locus.

• 出版日期2010-3