Water-compatible molecularly imprinted polymers for selective solid phase extraction of dencichine from the aqueous extract of Panax notoginseng

作者：Ji, Wenhua; Xie, Hongkai; Zhou, Jie; Wang, Xiao^*; Ma, Xiuli; Huang, Luqi

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2016, 1008: 225-233.

DOI：10.1016/j.jchromb.2015.11.053

• Molecularly imprinted polymers
• HPLC
• Natural products
• Dencichine
• Panax notoginseng

Specific molecularly imprinted polymers for dencichine were developed for the first time in this study by the bulk polymerization using phenylpyruvic acid and in-tyrosine as multi-templates. The photographs confirmed that molecularly imprinted polymers prepared using N,N'-methylene diacrylamide as crosslinker and glycol dimethyl ether as porogen displayed excellent hydrophilicity. Selectivity, adsorption isotherm and adsorption kinetics were investigated. The sample loading-washing-eluting solvent was optimized to evaluate the property of molecularly imprinted solid phase extract. Compared with LC/WCX-SPE, water-compatible molecularly imprinted solid phase extraction displayed more excellent specific adsorption performance. The extracted dencichine from Panax notoginseng with the purity of 98.5% and the average recovery of 85.6% (n = 3) was obtained.

• 出版日期2016-1-1
• 单位中国中医科学院; test; 齐鲁工业大学; 山东农业大学; 中医学科学院