Astragaloside IV Attenuates Lipolysis and Improves Insulin Resistance Induced by TNF alpha in 3T3-L1 Adipocytes

作者：Jiang, Boren; Yang, Ying; Jin, Hua; Shang, Wenbin; Zhou, Libin; Qian, Lei; Chen, Mingdao^*

来源：Phytotherapy Research, 2008, 22(11): 1434-1439.

DOI：10.1002/ptr.2434

• astragaloside IV
• 3T3-L1 adipocytes
• MAPK
• TNF alpha
• lipolysis
• perilipin

Increased circulating free fatty acid (FFA) concentrations have been demonstrated to potentially link obesity, insulin resistance and cardiovascular diseases. Astragaloside IV (AS-IV) is a saponin which is widely used in traditional Chinese medicine to treat type 2 diabetes and cardiovascular diseases. The purpose of the present study was to examine the effects of AS-IV on the lipolysis and insulin resistance induced by tumor necrosis factor-alpha (TNF alpha) in cultured 3T3-L1 adipocytes. TNFa promotes lipolysis in mammal adipocytes via the mitogen activated protein kinase (MAPK) family resulting in reduced expression/function of perilipin. Application of AS-IV inhibited TNF alpha-induced accelerated lipolysis in a dose-dependent manner, which was compatible with suppressed phosphorylation of ERK1/2 and reversed the downregulation of perilipin. Moreover, TNFa induced downregulation of key enzymes in lipogenesis, including LPL, FAS and GPAT, were also attenuated by AS-IV. Further studies showed that AS-IV improved TNFa-induced insulin resistance in 3T3-L1 adipocytes. This study provides the first direct evidence of the antilipolytic action of AS-IV in adipocytes, which may allow this agent to decrease the circulating FFA levels, thus increase insulin sensitivity and treat cardiovascular diseases.

• 出版日期2008-11
• 单位上海市闵行区中心医院; 上海交通大学