Microbiome analysis and bacterial isolation from Lejia Lake soil in Atacama Desert

作者：Mandakovic Dinka; Maldonado Jonathan; Pulgar Rodrigo; Cabrera Pablo; Gaete Alexis; Urtuvia Viviana; Seeger Michael; Cambiazo Veronica; Gonzalez Mauricio^*

来源：Extremophiles, 2018, 22(4): 665-673.

DOI：10.1007/s00792-018-1027-6

• Atacama Desert
• Microbiome
• Soil extract medium (SEM)
• Isolation and characterization

As a consequence of the severe climatic change affecting our entire world, many lakes in the Andes Cordillera are likely to disappear within a few decades. One of these lakes is Lejia Lake, located in the central Atacama Desert. The objectives of this study were: (1) to characterize the bacterial community from Lejia Lake shore soil (LLS) using 16S rRNA sequencing and (2) to test a culture-based approach using a soil extract medium (SEM) to recover soil bacteria. This extreme ecosystem was dominated by three phyla: Bacteroidetes, Proteobacteria, and Firmicutes with 29.2, 28.2 and 28.1% of the relative abundance, respectively. Using SEM, we recovered 7.4% of the operational taxonomic units from LLS, all of which belonged to the same three dominant phyla from LLS (6.9% of Bacteroidetes, 77.6% of Proteobacteria, and 15.3% of Firmicutes). In addition, we used SEM to recover isolates from LLS and supplemented the culture medium with increasing salt concentrations to isolate microbial representatives of salt tolerance (Halomonas spp.). The results of this study complement the list of microbial taxa diversity from the Atacama Desert and assess a pipeline to isolate selective bacteria that could represent useful elements for biotechnological approaches.

• 出版日期2018-7