Stability of rat models of fluid percussion-induced traumatic brain injury: comparison of three different impact forces

作者：Lin, Yun-peng; Jiang, Rong-cai; Zhang, Jian-ning^*

来源：Neural Regeneration Research, 2015, 10(7): 1088-1094.

DOI：10.4103/1673-5374.160100

• nerve regeneration
• traumatic brain injury
• fluid percussion
• impact force
• pressure curve
• head fixed
• impact peak
• animal models
• neural regeneration

Fluid percussion-induced traumatic brain injury models have been widely used in experimental research for years. In an experiment, the stability of impaction is inevitably affected by factors such as the appearance of liquid spikes. Management of impact pressure is a crucial factor that determines the stability of these models, and direction of impact control is another basic element. To improve experimental stability, we calculated a pressure curve by generating repeated impacts using a fluid percussion device at different pendulum angles. A stereotactic frame was used to control the direction of impact. We produced stable and reproducible models, including mild, moderate, and severe traumatic brain injury, using the MODEL01-B device at pendulum angles of 6 degrees, 11 degrees and 13 degrees, with corresponding impact force values of 1.0 +/- 0.11 atm (101.32 +/- 11.16 kPa), 2.6 +/- 0.16 atm (263.44 +/- 16.21 kPa), and 3.6 +/- 0.16 atm (364.77 +/- 16.21 kPa), respectively. Behavioral tests, hematoxylin-eosin staining, and magnetic resonance imaging revealed that models for different degrees of injury were consistent with the clinical properties of mild, moderate, and severe craniocerebral injuries. Using this method, we established fluid percussion models for different degrees of injury and stabilized pathological features based on precise power and direction control.

• 出版日期2015-7
• 单位天津医科大学; 天津市环湖医院