Molecular epidemiology of hepatitis B virus genotypes in Pakistan

作者：Alam Muhammad Masroor; Zaidi Sohail Zahoor^*; Malik Salman Akbar; Shaukat Shahzad; Naeem Asif; Sharif Salmaan; Angez Mehar; Butt Javed Aslam

来源：BMC Infectious Diseases, 2007, 7: 115.

DOI：10.1186/1471-2334-7-115

Background: Eight genotypes of Hepatitis B virus designated A-H, have been known but in Pakistan, no such data is available on the prevalent HBV genotypes. Therefore, the subject study was conducted to determine HBV genotypes in the indigenous Pakistani population.
Methods: A total of 690 individuals were enrolled for HBV screening with EIA and nested PCR. Positive samples were further analyzed to determine HBV genotypes (A-F) by multiplex-PCR using type specific primers.
Results: 110 (15.94%) individuals were positive for HBV, including 64% males and 36% females. Out of these, 66 samples (65.34%) were classified into genotype D, 27 (26.73%) were of genotype B while 5(4.95%) had genotype A. In 3 (2.98%) samples, multiple genotypes were detected (genotype A+B; 2(1.99%) and genotypes B+D; 1(0.99%). Nine (8.18%) samples remained untyable.
Conclusion: In Asia, genotypes B and C are the most prevalent but our study reveals that genotype D is predominant and HBV infection constitutes a significant health problem in Pakistan.

• 出版日期2007-10-8
• 单位河北医科大学