Multi-Pathways-Mediated Propagations of Intercellular Calcium Waves in Microglial Cells

作者：Wu Xian; Pan Lei-Ting^*; Liu Ying; Xing Meng-Xi; Zhang Xin-Zheng; Xu Jing-Jun

来源：Progress in Biochemistry and Biophysics, 2014, 41(2): 179-184.

DOI：10.3724/SP.J.1206.2013.00007

• BV-2 microglial cells
• intercellular calcium waves
• gap junction
• paracrine
• TNTs-like structure

In the present work, we applied mechanical stimulus to study the propagations of intercellular calcium waves among BV-2 microglial cells by the fast fluorescence microscopy imaging system in vitro. The results showed that calcium signaling responded to mechanical stimulus and propagated to neighboring cells in isolated BV-2 microglial cells, suggesting that the paracrine pathway mediated intercellular calcium waves. Further data indicated that gap junction was involved in mechanically-induced intercellular calcium waves when the cells had physical contact with each other. More importantly, tunneling nanotubes (INTO-like structure between BV-2 microglial cells was found in vitro and mediated the propagation of intercellular calcium wave. Taken together, our studies demonstrated that three distinct pathways, including paracrine, gap junction and TNTs-like structures, could mediate the intercellular calcium waves in BV-2 microglial cells.

• 出版日期2014-2
• 单位南开大学