Differential effects of two-pore channel protein 1 and 2 silencing in MDA-MB-468 breast cancer cells

作者：Jahidin Aisyah H; Stewart Teneale A; Thompson Erik W; Roberts Thomson Sarah J; Monteith Gregory R

来源：Biochemical and Biophysical Research Communications, 2016, 477(4): 731-736.

DOI：10.1016/j.bbrc.2016.06.127

• Breast cancer
• Calcium
• Calcium signaling
• Epidermal growth factor
• Two-pore channel
• Vimentin

Two-pore channel proteins, TPC1 and TPC2, are calcium permeable ion channels found localized to the membranes of endolysosomal calcium stores. There is increasing interest in the role of TPC-mediated intracellular signaling in various pathologies; however their role in breast cancer has not been extensively evaluated. TPC1 and TPC2 mRNA was present in all non-tumorigenic and tumorigenic breast cell lines assessed. Silencing of TPC2 but not TPC1 attenuated epidermal growth factor-induced vimentin expression in MDA-MB-468 breast cancer cells. This effect was not due to a general inhibition of epithelial to mesenchymal transition (EMT) as TPC2 silencing had no effect on epidermal growth factor (EGF)-induced changes on E-cadherin expression. TPC1 and TPC2 were also shown to differentially regulate cyclopiazonic acid (CPA)-mediated changes in cytosolic free Ca2+. These findings indicate potential differential regulation of signaling processes by TPC1 and TPC2 in breast cancer cells.

• 出版日期2016-9-2