A photosensitive surface capable of inducing electrophysiological changes in NG108-15 neurons

作者：Huang Fei; Bladon John; Lagoy Ross C; Shorrock Peter N Jr; Hronik Tupaj Marie; Zoto Christopher A; Connors Robert E; McGimpsey W Grant; Molnar Peter; Lambert Stephen; Rittenhouse Ann R^*; Lambert Christopher R

来源：Acta Biomaterialia, 2015, 12: 42-50.

DOI：10.1016/j.actbio.2014.10.023

• Current clamp
• Neuronal stimulation
• Retinal prosthetic
• Self-assembled monolayer (SAM)
• Voltage clamp

Retinal prostheses promise to be a viable therapy for many forms of blindness. Direct stimulation of neurons using an organic light-sensitive, self-assembled monolayer surface offers a simple alternative to conventional semiconductor technology. For this purpose we have derivatized an indium tin oxide (ITO) substrate with the photosensitive dye, NK5962, using 3-(aminopropyl)trimethoxysilane (APTMS) as cross-linker. The surface was characterized through contact angle goniometry, electrochemical impedance spectroscopy, grazing angle infrared and ultraviolet visible spectrophotometry. NG108-15 neurons were grown on the ITO-APTMS-NK5962 surface and neural responses from electrical stimulation vs. photostimulation through the ITO-APTMS-NK5962 surface were measured using patch clamp electrophysiology. Under these conditions, photostimulation of depolarized cells caused an approximate 2-fold increase in voltage-gated sodium (Na+) current amplitude at a membrane potential of -30 mV. Our results demonstrate the feasibility of stimulating neurons, grown on light-sensitive surfaces, with light impulses, which ultimately may facilitate the fabrication of a simple, passive retinal prosthetic.

• 出版日期2015-1-15