A novel killer protein from Pichia kluyveri isolated from an Algerian soil: purification and characterization of its in vitro activity against food and beverage spoilage yeasts

作者：Labbani Fatima Zohra Kenza^*; Turchetti Benedetta; Bennamoun Leila; Dakhmouche Scheherazad; Roberti Rita; Corazzi Lanfranco; Meraihi Zahia; Buzzini Pietro

来源：Antonie van Leeuwenhoek, 2015, 107(4): 961-970.

DOI：10.1007/s10482-015-0388-4

• Yeast killer protein
• Pichia kluyveri
• Susceptibility testing
• Spoilage yeasts
• Minimum inhibitory concentrations (MICs)

A novel killer protein (Pkkp) secreted by a Pichia kluyveri strain isolated from an Algerian soil was active against food and beverage spoilage yeasts of the genera Dekkera, Kluyveromyces, Pichia, Saccharomyces, Torulaspora, Wickerhamomyces and Zygosaccharomyces. After purification by gel filtration chromatography Pkkp revealed an apparent molecular mass of 54 kDa with SDS-PAGE. Minimum inhibitory concentrations (MICs) of purified Pkkp exhibited a high in vitro activity against Dekkera bruxellensis (MICs from 64,000- to 256,000-fold lower than that exhibited by potassium metabisulphite) and Saccharomyces cerevisiae (MICs from 32,000- to 64,000- fold lower than potassium sorbate). No in vitro synergistic interactions (calculated by FIC index - I pound FIC) were observed when Pkkp was used in combination with potassium metabisulphite, potassium sorbate, or ethanol. Pkkp exhibited a dose-response effect against D. bruxellensis and S. cerevisiae in a low-alcoholic drink and fruit juice, respectively. The results of the present study suggest that Pkkp could be proposed as a novel food-grade compound useful for the control of food and beverage spoilage yeasts.

• 出版日期2015-4