A Convenient Alternative to MALDI and ESI

作者：Trimpin Sarah^*; Wang Beixi; Lutomski Corinne A; El Baba Tarick J; Harless Bryan M

来源：Spectroscopy, 2016, 31(3): 53-61.

Ionization of small, large, volatile, and nonvolatile compounds with charge states nearly identical to electrospray ionization are produced from a solid matrix or solution with high sensitivity utilizing the vacuum inherent with any mass spectrometer. With the proper matrix, analytes can be analyzed from ambient conditions or by direct introduction into vacuum. The ion source is simply the inlet to the mass analyzer. The new ionization methods have been interfaced with liquid chromatography, used for imaging tissue at atmospheric pressure or in vacuum, demonstrated for high-throughput analyses, applied for fast defect analysis, and shown to be compatible with electron transfer dissociation and ion mobility spectrometry mass spectrometry. With the proper matrix, even large proteins are converted to gas-phase multiply charged ions without application of an external source of energy other than subatmospheric pressure. This latter method has great potential for extending mass spectrometry to areas such as clinical analysis where cost, robustness, and simplicity are important assets.

• 出版日期2016-3