Antioxidant enzyme gene expression in response to copper stress in Withania somnifera L.

作者：Rout Jyoti R^*; Sahoo Santi L

来源：Plant Growth Regulation, 2013, 71(1): 95-99.

DOI：10.1007/s10725-013-9806-7

• Antioxidant enzymes
• Copper stress
• Gene expression
• Withania somnifera L.

Production of reactive oxygen species is related with a number of environmental stress impacts in plants. The major impact is caused by the increasing concentration of metals in atmosphere. To understand the functions of antioxidant enzymes during metal (Cu) stress, we investigated the level of expression in catalase (CAT), peroxidase (POX) and superoxide dismutase (SOD) from leaf and root samples of Withania somnifera L. Healthy and uniformly in vitro grown plants were exposed to different concentrations (0, 25, 50, 100 and 200 mu M) of Cu for 7 days in full-strength MS (Murashige and Skoog) liquid medium. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis was performed by taking three pairs of genes of CAT (RsCat, Catalase1, Cat1), SOD (SodCp, TaSOD1.2, MnSOD) and POX (Peroxidase, spa15, Apx4). Only one gene from CAT (RsCat) and SOD (MnSOD) exhibited high levels of gene expression under Cu excess, but no gene from POX was expressed satisfactory. This experiment supported a difference in gene expression in response to Cu treated and control tissue samples of plants. The techniques and primers used here to demonstrate such changes in Cu treated plant of W. somnifera can be used to examine the other medicinal plants in related to oxidative stress.

• 出版日期2013-9