Multi-target siRNA based on DNMT3A/B homologous conserved region influences cell cycle and apoptosis of human prostate cancer cell line TSU-PR1

作者：Du, Yue-feng; Liang, Liang; Shi, Ying; Long, Qing-zhi; Zeng, Jin; Wang, Xin-yang; He, Da-lin^*

来源：Genetics and Molecular Biology, 2012, 35(1): 164-171.

DOI：10.1590/S1415-47572012005000021

• prostate cancer
• DNA methylation
• DNMT3
• RNA interference

Abnormal genome hypermethylation participates in the tumorigenesis and development of prostate cancer. Prostate cancer cells highly express DNA methyltransferase 3 (DMNT3) family genes, essential for maintaining genome methylation. In the present study, multi-target siRNA, based on the homologous region of the DNMT3 family, was designed for the in vitro investigation of its effects on the proliferation, migration, and invasion of TSU-PR1 prostate cancer cells. The consequential cell-cycle derangement, through DNMT3A/B or only DNMT3B silencing, was partially efficient, without affecting apoptosis. DNMT3A silencing had absolutely no effect on changing TSU-PR1 cell biological behavior. Hence, DNMT3B alone apparently plays a key role in maintaining the unfavorable behavior of prostate-cancer cells, thereby implying its potential significance as a promising therapeutic target, with DNMT3A simply in the role of helper.

• 出版日期2012
• 单位西安交通大学; 华中科技大学