Anti-obesity effects of zeaxanthin on 3T3-L1 preadipocyte and high fat induced obese mice

作者：Liu, Meihong; Liu, Huimin; Xie, Jiahan; Xu, Qian; Pan, Cong; Wang, Jiaojiao; Wu, Xi; Sanabil; Zheng, Mingzhu; Liu, Jingsheng^*

来源：Food & Function, 2017, 8(9): 3327-3338.

DOI：10.1039/c7fo00486a

• ACTIVATED PROTEIN-KINASE
• WHITE ADIPOSE-TISSUE
• DIET-INDUCED OBESITY
• INHIBITS ADIPOGENESIS
• OXIDATIVE STRESS
• DOWN-REGULATION
• OXIDIZED LDL
• LUTEIN
• ACID
• ACCUMULATION

Zeaxanthin, a type of carotenoid, has been proven to exhibit anti-lipogenesis effect; however, the detailed mechanism of this effect is less known. Herein, we evaluated the effects of zeaxanthin on the inhibition of adipogenesis in 3T3-L1 adipocytes and obesity in high-fat diet fed C57BL/6J mice. Zeaxanthin significantly decreased the intracellular lipid content in a dose-dependent manner (5-15 mu M) in adipocytes without causing cytotoxicity. In high-fat-diet-induced obese mice, oral administration of 20 mg kg(-1) zeaxanthin attenuated the progression of obesity and improved dyslipidemia. It exhibits an anti-adipogenic effect via down-regulating the transcriptional factors and adipocyte-specific genes involved in adipogenesis, both in vitro and in vivo. Furthermore, zeaxanthin treatment reversed the MDI (0.5 mM 3-isobutyl-1-methylxanthine, 1.0 mu M dexamethasone, and 1.0 mu g mL(-1) insulin) and HFD (high-fat diet)-induced inhibition of AMPK phosphorylation in adipocytes and epididymal adipose tissues, respectively, thereby modulating the energy metabolism. These results indicated that zeaxanthin plays anti-adipogenic and anti-obesity roles by inducing AMPK activation, inhibiting lipogenesis, and decreasing intracellular lipid content, adipocyte size, and adipose weight.

• 出版日期2017-9-1
• 单位吉林农业大学