An improvement on mass spectrometry-based epigenetic analysis of large histone-derived peptides by using the Ionization Variable Unit interface

作者：Fukuda Tetsuya; Hike Hiroshi; Usui Fumihiko; Bando Yasuhiko; Nishimura Toshihide; Kodama Tatsuhiko; Kawamura Takeshi^*

来源：Analytical Biochemistry, 2015, 486: 14-16.

DOI：10.1016/j.ab.2015.06.002

• Charge state conversion
• Multiply protonated peptides
• Collision-induced dissociation (CID)
• Epigenetics
• Mass spectrometry

Highly protonated histone-derived peptides impede a sufficient mass spectrometry (MS)-based epigenetic analysis because their relatively low mix, due to a high degree of proton addition to peptides, would make it difficult to analyze the resulting complex MS/MS spectra. To reduce the degree of protonations, we have developed a new interface, the Ionization Variable Unit (IVU), in which peptides are ionized under a vaporized organic solvent. It is demonstrated that the doubly charged histone tail H2B peptide, PEPAKSAPAPKKGSKKAVTKAQKK (m/z 1238.243, +2), which was not detectable before, can be detected by using the IVU interface and sequenced.

• 出版日期2015-10-1